The gel is then blotted on a nylon membrane or a nitrocellulose filter. The RNA samples are separated according to their size on an agarose gel. RNA is isolated from several biological samples (e.g., various tissues, various developmental stages of same tissue, etc.) Wear gloves use specially treated plastics and glassware to avoid accidental introducing ribonuclease to extraction prep.Īddition of di-ethyl-pyro-carbonate (DEPC) inhibits ribonuclease activity and also baking at high temperature destroys ribonuclease activity (only useful for treating heat resistant equipment like glassware). RNA is extracted from the cells of interest, but precautions must be taken to avoid degradation of single-stranded RNA by ribonuclease (RNase), which is found on the skin and on glassware. RNA molecules are separated by size and detected on a membrane using a hybridization probe with a base sequence complementary to all, or a part, of the sequence of target RNA. It is one of the key techniques in molecular biology, its principal aim being the measurement of RNA (in particular mRNA). Northern blotting is a simple extension of Southern blotting, and derives its name from the earlier technique. Used in DNA fingerprinting, genetic engineering and forensic science for tests as under:ĭ. To detect certain cancers and genetic diseases, such as:Ĩ. To identify related DNA sequence in the genome and to determine if there is a gene family (a group of similar genes).ħ. To determine the number of copies of a particular DNA sequence presented in the genome of an organism.Ħ. To identify gene mutation, deletion, duplication, and gene rearrangement involved in diseases.ĥ. To analyse the genetic patterns in an organism’s DNA.Ĥ. Used in gene discovery and gene mapping.ģ. To identify a single gene among thousands of fragments of DNA and to detect sequences of DNA in an organism’s genome.Ģ. Whether re-arrangements have occurred during the cloning process. By performing the digestion with different endonucleases, or with combinations of endonucleases, it is possible to obtain a restriction map of the gene, i.e., an idea of restriction enzyme sites in and around the gene which will assist in attempts to clone the gene.Ĥ. Whether recognition sites for particular restriction endonucleases are present in the gene. The degree of similarity between the chromosomal gene and the probe sequence.ģ. Whether a particular gene is present and how many copies are present in the genome of an organism.Ģ. We can get following information from the technique of Southern blotting ġ. Information Obtained from Southern Blotting : If the probe has been labelled with 32P, it will be radioactive, and the sheet will show a band of radioactivity where the probe is hybridized with the complementary fragment. Next, a probe consisting of purified, single-stranded DNA corresponding to a specific gene (or mRNA transcribed from that gene) is poured over the sheet.Īny fragment that has a nucleotide sequence complementary to the probe’s sequence will hybridize (base pair) with the probe. The double-stranded helix of each DNA fragment is then denatured into single strands by making the pH of gel basic, and the gel is “blotted” with a sheet of nitrocellulose, transferring some of the DNA strands to the sheet. In this procedure, called a Southern blot, DNA from the sample is cleaved into restriction fragments with a restriction endonuclease, and the fragments are spread apart by gel electrophoresis. If we want to detect the presence of a specific sequence in our mixed DNA sample then we will accordingly design the probe which will have complementary sequence to our target sequence. In this technique we exploit the property of a radio-labelled probe with the single stranded DNA. This technique was invented in 1975 by E.M. Southern blot hybridization detects target DNA fragments that have been size-fractionated by gel electrophoresis. The following points highlight the top seven blotting techniques used in detection of DNA.
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The problem with the GIF type is that those with transparent backgrounds need to be treated differently to those without. This ability is essential for my application which uploads any of GIF, JPEG or PNG and simultaneously creates a thumbnail of identical image type and identical filename (full size and thumbnail versions being stored in different folders).Īfter uploading and moving the image in the usual way, a switch($image_type) statement ensures that the optimum code is used to generate the thumbnail regardless of image type. If there some errors, I hope you could understood the idea.Īfter much devious mindbending, I have found a way to test any GIF for presence of background transparency. I used (nearly) the following code to make resized GIF images trnsparent only when the transparency is needed: The solution is not to use some default transparent color and to leave the resized image without transparent color (the same as original GIF). The problem is, that the color you force to be transparent can be used in the original GIF as opaque and you will loose that color in resized image. ![]() But sometimes the transparent color in GIF images can be not set. A good example of forced transparency in resized GIF image was given by markglibres at yahoo dot com 2 02:48. Pay attention, that some GIF images may not include a transparent color. Imagecolortransparent ( $new_image, $transparencyIndex ) Imagefill ( $new_image, 0, 0, $transparencyIndex ) $transparencyIndex = imagecolorallocate ( $new_image, $transparenc圜olor, $transparenc圜olor, $transparenc圜olor ) $transparenc圜olor = imagecolorsforindex ( $image_source, $transparencyIndex ) $image_source = image resource identifier returned by imagecreatefromjpeg, imagecreatefromgif and imagecreatefrompng. $new_image = image resource identifier such as returned by imagecreatetruecolor(). NOTE: Transparency is only supported on GIF and PNG files. I've made a very simple script that will retain transparency of images especially when resizing. Getting Started Introduction A simple tutorial Language Reference Basic syntax Types Variables Constants Expressions Operators Control Structures Functions Classes and Objects Namespaces Enumerations Errors Exceptions Fibers Generators Attributes References Explained Predefined Variables Predefined Exceptions Predefined Interfaces and Classes Predefined Attributes Context options and parameters Supported Protocols and Wrappers Security Introduction General considerations Installed as CGI binary Installed as an Apache module Session Security Filesystem Security Database Security Error Reporting User Submitted Data Hiding PHP Keeping Current Features HTTP authentication with PHP Cookies Sessions Dealing with XForms Handling file uploads Using remote files Connection handling Persistent Database Connections Command line usage Garbage Collection DTrace Dynamic Tracing Function Reference Affecting PHP's Behaviour Audio Formats Manipulation Authentication Services Command Line Specific Extensions Compression and Archive Extensions Cryptography Extensions Database Extensions Date and Time Related Extensions File System Related Extensions Human Language and Character Encoding Support Image Processing and Generation Mail Related Extensions Mathematical Extensions Non-Text MIME Output Process Control Extensions Other Basic Extensions Other Services Search Engine Extensions Server Specific Extensions Session Extensions Text Processing Variable and Type Related Extensions Web Services Windows Only Extensions XML Manipulation GUI Extensions Keyboard Shortcuts ? This help j Next menu item k Previous menu item g p Previous man page g n Next man page G Scroll to bottom g g Scroll to top g h Goto homepage g s Goto search ![]() Create dynamic presentations - easily incorporate images and hyperlinks into XMind's stylish one-click presentation mode.Simply drop and drag any task and order it by priority and start and stop time. Keep a time table - XMind Pro 2013 Gantt view organizes tasks related to the project.Check out some publicly hosted maps to see how it all works. Share with the group - XMind Pro 2013 maps can be set to public or private and hosted for online viewing.Stay focused, while doing the detail work - XMind Pro 2013 allows topic points to become the main points of their own maps to quickly get into the nitty gritty, without losing sight of the overall focus.Map complex ideas and related subtopics - XMind Pro 2013 allows for mapping information on screen exactly as it's mapped in your mind.Want to learn A LOT more? Check out the detailed feature list, any or all of the comprehensive video demos, or take a spin around an actual XMind Pro 2013 mind map. ![]() No more getting everyone on the same page - now you can make sure that everyone is of the same mind. XMind Pro 2013 lets you share maps with a group online, publicly or privately, and updates changes as needed.Ĭheck out some of the mind maps created by other XMind users!Īnd when it's finally time to present the ideas on the map, the XMind Pro 2013 one-click presentation mode will blow them away! XMind Pro 2013 walks through your mind map as you click, revealing one detail at a time, delving further into your subject, staying focused and centered in the screen. All subtopics can become the main topics of their own maps, allowing you to endlessly delve into the minutiae of a topic, while leaving a quick trail back to the main subject. Hong Kong based Xmind offers their diagramming tool featuring a range of templates. so the information is organized in the most logical way - the way you thought of it. XMind Pro 2013 generates mind maps in a variety of structures: fishbone, linear, tree etc. From the appearance, Xmind (2022) is modern and chic while XMind 8 Pro looks classic and grounded. Xmind (2022) is subscription-based, while XMind 8 Pro lifetime license key. XMind Pro 2013 provides a slick, sophisticated and intelligently organized forum for you and your team to work together. Xmind (2022) and XMind 8 Pro are both our desktop mind mapping software, but their licensing modes are different and also support some different features. But you can't make a presentation out of legal pads, post-it notes and bar napkins. You need flexibility, something more nuanced, more human. functies zoals het exporteren van mindmaps naar PDF-, Powerpoint- of Word-bestanden moet de betaalde versie van XMind aangeschaft worden: XMind Pro. XMind Pro 8 Dystrybucja i Sprzeda Oprogramowania (Think-Cell, JetBrains, Total Commander i mnstwo innych). Sometimes a slideshow just doesn't cut it. Also, this offer is for 2 XMind Pro 2013 licenses including 2 years of upgrades.Ĭomplex ideas require complex explanations - illustrations, details, tangential but essential strings of logic. Please note folks, you will receive your XMind license information in the next couple of business days. When editing, you can quickly open, close and switch views with just one click. ![]() The German health-care market can be attractive, especially for investment in hospitals, nursing homes, rehabilitation facilities and medical-care centres.Germany is reinventing its auto industry from the ground up, especially in the areas of electric and hybrid drivetrains, autonomous driving, emission controls and smart technologies related to the sector.The German market is also open to entrepreneurial investment in practically all areas, with good opportunities in several sectors: Key export sectors for Canada include aerospace, advanced manufacturing, automotive products, life sciences, information and communications technologies, and agri-food. In 2020, our Germany-bound exports were worth $6.37 billion, while we bought $17.27 billion in German goods. Germany is Canada’s largest EU export market and our sixth-largest trading partner globally. Germany’s reputation for productivity, quality and innovation is founded largely on the country’s highly skilled workforce, which boasts exceptional levels of educational attainment.Close co-operation between industry and major research institutions helps transform new ideas into products for the world market. The country is a world leader in high-technology R&D and offers an ideal environment for innovation.It’s also a reliable investment environment with strong protections for intellectual property. Germany offers powerful R&D and investment incentive packages that can help reimburse a project’s costs to the investor.It’s the largest consumer market in Europe, measured in both population size and purchasing power.Germany is strategically located in the centre of Europe, with excellent logistics capabilities that provide rapid access to all European markets and suppliers.Establishing a business presence in Germany can provide a Canadian firm with a range of other advantages: ![]() The Comprehensive and Economic Trade Agreement between Canada and the European Union ( CETA) already gives Canadian companies relatively easy access to the EU market directly from Canada. Robust economic growth is expected to resume in 2021 and beyond. The German economy expanded steadily after the end of the 2009 economic crisis, and despite its growth faltering during with the onset of the global pandemic, Germany’s 5% contraction in gross domestic product (GDP) in 2020 was one of the smallest in Europe. “Thanks to the country’s intensive research and innovation, together with its ability to attract large amounts of FDI (foreign direct investment), it boasts the largest economy in Europe and the fourth-largest worldwide.” “Germany has one of the world’s most advanced economies and is a global economic powerhouse,” says EDC’s Klaus Houben, senior regional manager for Europe. Free registration gives you fast access to a wide range of information and resources. This interactive tool can help you understand your export and foreign investment risks in 50 markets. Export Development Canada’s (EDC) Country Risk Quarterly In this article, we highlight Germany, Peru and Vietnam and examine the economic environment, trade relationship, export potential and market challenges of doing business with these countries. With global trade heating up, there are a number of export opportunities worth exploring for Canadian companies. ![]() It's like 13 for a combo that comes with a burger, fries and a medium drink. Track calories, carbs, fat, sodium, sugar & 14 other nutrients. ![]() Their Ice Cream is the best in town, and their burger combos are scrumptious. Instead of freshly squeezed lemon juice and sugar, you can make this with frozen lemon concentrate and vanilla ice cream. LaRocco Pizzas, Applebee's, A&W, Arby's B Brixx, Burgerville, Burger King, Ben & Jerry's, Ben & Jerry's C Chick-fil-A, Cafe Rio, California Tortilla, California Pizza Kitchen, Casey's D Del Taco, Dickey's Barbecue, Dairy Queen, Denny's, Doc Green's E Emerald City Smoothie, El Pollo Loco, Elephant Bar, East Side Mario's, Energy Kitchen F Freddy's Frozen Custard, Flame Broiler, Fuddruckers, Fazoli's, Fatburger G Gandolfo's, Good Times, Genghis Grill, Garbanzo Mediterranean Grill, Grandy's H Hardee's, Hungry Howie's Pizza, Hale and Hearty, Hometown Buffet, HuHot Mongolian Grill I Ikea, IHOP, Isaac's, In-N-Out, Il Vicino J Jugo Juice, Jet's Pizza, Jersey Mike's, Joe's Crab Shack, Jamba Juice K KFC, Kwik Trip, King Taco, Krystal, Keva Juice L Leeann Chin, LifeCafe, LaRosa's Pizzeria, Long John Silver's, Lenny's M Manhattan Bagel, Meatheads, Milestone's, Muscle Maker Grill, Macayo's N Noodles & Company, Noah's, Nathan's Famous, Nordstrom, Newk's Express Cafe O Old Chicago, Orange Leaf, Olive Garden, Ono Hawaiian BBQ, O'Charley's P Pollo Tropical, Pita Pit, Peet's Coffee & Tea, Pei Wei, Paradise Bakery Q Qdoba Mexican Grill, QuikTrip, QT Kitchens, Quiznos, R Raising Cane's, Rock Bottom, Red Lobster, Roberto's Mexican Food, Red Mango S Subway, Spaghetti Warehouse, Spring Creek Barbeque, Souplantation, Seasons 52 T Taco Time, The Chop House, Tasti D-Lite, The Varsity, Taco John's U Uno Chicago Grill, UFood Grill, W Waba Grill, Wendy's, Winger's, Which Wich, Wingstop Y Yogurtland, Yaya's, Yoshinoya, Yard House, Z Zippy's, Zpizza, Zoup!, Zaxby's, Zoe's Kitchen. 21 reviews 1 photo 3.5 Honestly, I love this place. Nutrition Facts Crinkle Cut French Fries, 6.0 oz Serving Size: 6 oz. Other Restaurants & Chains A Abner's, A.C. 790 cals Quantity Nutritional Facts Serving Size: 1 serving Serving Weight: 0g Nutrient Value Calories 790 kCal Total Carbs 89 g Net Carbs 89 g Fiber - Starch - Sugar - Sugar Alcohols - Protein - Fat - Monounsat. ![]() But there is something that stays true: One taste will turn any non-cheese eater into a full-blown dairy hoarder. Decades ticked past, as memories faded and Mary sold off her goats to neighboring farms and sold her company. The effeminate and gentle sweetness of the Cali goat danced delicate circles around the aggressive Frenchie goats of the Loire. When Robin Leach jetted off on those gilded yachts on Lifestyles of the Rich and Famous, there was always a wedge on hand. Deer Creek Cheddar Cheese from a small family run creamery in Sheboygan, Wisconsin. Thanks to the excessive affluence of creepy dudes wearing linen blazers with shoulder pads- Miami Vice, you gave us so much-and their trophy-bronzed wives, goat cheese became a status symbol for the rich and famous. It's the cheese that will make any grown woman damp in the panties after just one lick. After breeding the kids-the goats, not the daughters-she began crafting cheeses in the 80s, when the peak of the Cali cocaine heyday took hold, and roasted beets as a pizza topping were all the rage. The single mama of four daughters did what any working woman would do: She made the damn thing pop. ![]() The stony folks up at Cypress Grove have been cranking out this little nugget of all-natural goat's milk since the 70s, when Mary Keehn chased down some goats with a can of oats. It looks like a birthday cake, all pillowy and white, all virginal and lovely. The cheese that makes the most dapper Dan get a stiffy at first sight. Oysters' slippery vaginal-esque-ness got nothing on this goat-y hit. Humboldt Fog Cheese is named for the way it reminds one, reputedly, of morning fog in northern California.Man, this is the real aphrodisiac. Humboldt Fog was awarded first prize in 2002 by the American Cheese Society in Washington for “American Original Cheese.” Language Notes Humboldt Fog Cheese was created in approximately 1992 by Mary Keehn. As the larger size ages past 2 months the cheese will start to ooze under the rind, and the flavour gets stronger. The small sizes are allowed to mature 3 weeks the larger ones for a minimum of 4 weeks. ![]()
![]() I like orange drop because they look cool but I’m quite happy with Mylar Film as well. Players argue obsessively about one capacitor material -vs- another but I’ll completely bypass that argument, because it’s an artistic choice and not based on significant measurable differences in capacitance. Ultimately what is MOST important is the VALUE of the capacitor which is usually expressed in MICROFARADS, or uF. However, other factors affect the capacitance (such as the dielectric material) The material that is in between the plates affect many parameters of the capacitor.Ĭapacitors block DC voltage and only pass AC (something with a frequency). Large plates let lower frequencies through whereas smaller plates let only higher frequencies through. The voltage (a really REALLY small AC from a pickup) appears across the plate. There are 2 metal plates with a dielectric in between them. There are MANY different types of capacitor materials but they operate with the same basic concept. 0068 uF – I sometimes use this as a “trim” cap in parallel to fine tune the tone response, By itself it’s not all that useful because it frequency cutoff would be too high. It removes the very top frequencies at the upper range 010 uF – The lowest I use, however, very usable, but lots of treble left. 015 ♟ – Very mild cut actually sounds like a dark humbucker if all off, slightly tone modifying.) 022 ♟ – Very common, especially on Modern Stratocasters 033 ♟ – Less common – Darker, less treble quicker 047 ♟ – Probably as big as you’d want to go – Loses treble quick – lower rolloff – original stratocaster value ![]() 062 uF – MEGA dark, not sure what you’d do with this. TYPICAL VALUES In Micro Farads which is 1000 NF (nano farads) – uF means Micro Farads. The list below shows typical values and how they sound in general. In general the smaller the value, the more higher frequencies can get through. They filter out (shunt to ground) or block (if in series) frequencies. Importantly, a passive circuits do NOT add any bass, PERIOD. If the cap value was really small, the filter frequency would be so high that no audible signal would be affected. This is because the filter frequency shifts higher so more high frequency remains unfiltered. 015 or less) the less the treble is removed when in a normal tone circuit. The easiest way to think of a tone capacitor is in the typical tone circuit they are a HIGH FREQUENCY REMOVAL (OR BLOCKING Device in the case of a BassTamer) device. ![]() Tone Capacitors are the MOST misunderstood component in a guitar. ![]() The other neutrons either escape out or are absorbed in any other medium except uranium. It is possible to produce such conditions in which only or neutron, out of all the neutrons created in one fission reaction becomes the cause of further fission reaction. In this way, this process rapidly proceeds and in an infinitesimally small time a large amount of energy along with huge explosion produces, it is a representation of fission chain reaction If conditions are appropriate these neutrons produce fission in some more atoms of uranium. Out of this reaction, about three neutrons are emitted. Suppose that we have a definite amount of and a slow neutron originating from any source produces fission reaction in one atom of uranium. The process is called a fission chain reaction. By properly using these neutrons fission reaction can be produced in more uranium atoms such that a fission reaction can continuously maintain itself. We have observed that during fission reaction a nucleus of uranium-235 absorbs a neutron and breaks into two nuclei of almost equal masses besides emitting two or three neutrons. However, it not been observed that fission takes place very easily with the slow neutrons in uranium-235 and plutonium-239, and mostly these two are used for fission purposes. Fission reaction is not confined to uranium along it is possible in many other heavy elements. All of these products (fragments) are radioactive. Hence in the uranium fission reaction, several products may be produced. Two possible fission reactions of uranium are given below as an example: In fact, any of the two nuclei present the upper Horizontal part of binding energy that could be produced. The fission process of uranium does not always produce the same fragments (Ba, Kr). This means that an energy 235 × 0.9 = 211.5 MeV is given out in the fission of one uranium nucleus. Thus when a uranium nucleus breaks up, as a result of fission reaction, into barium and krypton, then energy at the rate of 0.9 MeV per nucleon is given out. This graph shows that the binding energy per nucleon is greatest for the middle elements of the periodic table and this bending energy per nucleon is a little less for the light or very heavy elements i.e., then nucleons in the light or very heavy elements are not so rigidly bound.įor example, the binding energy per nucleon for uranium is about 7.7 MeV and the products of the fission reaction of uranium, namely barium and krypton, have a total mass less than the mass of uranium equal to 8.5 -7.6 = 0.9 MeV per nucleon. ![]() It may be kept in mind that three is no difference between the sum of the mass and the charge number on both sides of the equation.įission reaction can be easily explained with the help of a graph. Here Q is the energy give out in this reaction, By comparing the total energy on the left side of the equation with total energy on the right side, we find that in the fission of one uranium nucleus about 200 MeV energy is given out. ![]() ![]() These all offer up a sense of rugged individuality, protection from the elements, and all-American build to be proud of. Here are some of Bumper Superstore’s favorite front bumpers. The bumpers your truck is equipped with straight off the factory line are good enough to pass line inspection but may not be fully trustworthy enough to handle what you want to throw at your truck when you are on an all-out adventure. Just like the misfortune of being situated deep in the throngs of a Baja race or the beautiful finish of Azusa Canyon without GPS or durable camping gear, you never want to drive a single mile of your favored terrain without the addition of a valuable and durable front bumper. Our vast selection of front bumpers for your truck are some of the most durable pieces of equipment we have ever run across, and they remain a very trusty and reliable alternative to tackle what’s situated in front of you in the canyon or on the tundra. Front bumpers mount directly to the frame of your truck which is the only way to guarantee a solid mount and extra strength. These aftermarket front bumper parts are built with passion from leading brands like Ranch Hand, Bodyguard, Iron Cross, Road Armor, ARB 4x4 and many more. Our selection of front bumper replacements are direct bolt on replacements that require no drilling during installation. Lowest Price for Ranch Hand, Iron Cross, Road Armor Shop now. At we have built a large selection of aftermarket front bumpers for you to check out. Shop 2011-2014 Chevy Silverado Winch Bumper 2500/3500 HD at. Fits All Trim Levels.A front bumper can make or break the style and most importantly the safety of your rig and family. Silverado 2500 & 3500 Models, Fits All Trim Levels (cat eye generation). Remove your factory bumper/mounts, and our product directly bolts onto your vehicle’s factory bumper mounting locations. Most installations are completed in a few hours or less with basic hand tools the bumpers directly bolt onto your vehicle. Simple bolt-on installation, no welding or grinding is needed to install our Octane Series bumpers. There are also two 3” Cube lights in each wing of the bumper, totaling four Cube lights in the front bumper. Both light mounting options are included in the bumper, and the choice is yours which look you prefer to use. This bumper also includes two center section lighting options for the bumper- You can either choose to install a 30” curved or straight dual row LED Lightbar or install eight 3” LED Cube lights. All of Chassis Unlimited’s products are designed & manufactured in Livermore California This modular design allows us to offer an extreme tight contour of the vehicle, as well as a product design & extreme strength that will last a lifetime. Chevy K10, K20, K30 & Chevy Suburban Custom USA Front Winch 3/16' Plate Bumper -Square Body Front (Non-Winch Model Available) PRECISION WELDED MODEL -High Quality USA OPTIONS AVAILABLE Grizzly Metalworks 995. The Octane series is designed as a 3-Piece modular bumper, the outer bumper wings bolt to the center core section of the bumper. The Octane series bumpers bring an entire new style to the bumper market and offer an industry first low-profile fitment with aggressive styling. Our Non-Winch Bumper features room for a 30" curved or straight dual row light bar, HD 1” thick recovery points, and mounts up to 4 LED Cube lights in the outer wings of the bumper. 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